Introduction


Biomedical Proteomics Facility (BPF)

The Proteomics Core Facility is located at the Pathology Department and the Microbiology and Immunology Department. The facility is equipped with Isoelectric Focusing (IEF), two-dimensional gel electrophoresis   (2D-GE) and two separate data stations dedicated for proteomics data processing, database searching, and generation of reports. Several popular software packages are installed on the data station including PD-quest software and Panthere system. For protein and peptide fractionation, the Facility maintains a Rotafor, Biorad that performs IEF based fractionation in solution.

 

The Proteomics Core Facility is a fee for service facility supported by the RCMI BPF grant. The facility offers service to all researchers in the region, with priority given to users from the UCC. Routine service includes  IEF and 2D-GE electrophoresis molecular weight determination of intact proteins by LC/MS and protein identification through tryptic digestion, LC/MS/MS analysis and database searching.  Samples can be submitted as cells, tissue and lyophilized powders, solutions, or 2D gel spots.  


Sample Requirements:
-Total protein: > 200μg/sample, low salt (< 50 mM), without ionic detergents
-Cells: > 5×10-6 cells/sample, 3x washed and centrifuged, 4°C, pellet flash frozen
-Tissue: >10 mg blood-free tissue, washed by PBS, 4°C, flash frozen

Please refer to the Sample Preparation 2D-GE for detailed information.

The facility also welcomes collaborative research on projects beyond routine services. Please contact the facility director Dr. Nawal Boukli to initiate a collaborative research.
 

Contact
Dr. Nawal Boukli
Associate Professor 
Proteomics Facility Director 
Universidad Central del Caribe School of Medicine 
Department of Microbiology and Immunology 
Room 155. Urb. Santa Juanita Ave. Laurel 
Bayamon, Puerto Rico 00956 
Tels: (787) 798-3001 Ext. 2080 (office)     
        (787) 798-3001 Ext. 2090 (lab)
Fax:  (787) 740-4300 
Email: nawal.boukli@uccaribe.edu