High Quality Services & Fees

Here at the BPF, we offer a myriad of services related to quantitative proteomics. Such services are as follows:

  • 2D gel production (Material analysis service)
    Up to 12 gels.
  • Analysis of protein distribution of 2D gel (Material analysis service)
  • Tandem Mass Tagging (TMT) is a quantitative proteomic approach which allows the comparison of protein levels in up to 10 different samples in a single experiment. It is applicable to any sample type. With this approach it is possible to identify and quantitate thousands of proteins in a single experiment. Samples for TMT analysis are labelled within the facility, fractionated and analysed by nano-LC MSMS mass spectrometer.
  • Desalting (Material processing service)
  • Mass spectrometry analysis service (Material analysis service)
    Mass spectrometry analysis include: ESMS on Q-TOF Micro, LCMS, MALDI-TOF, MALDI (Linear=Reflection), MALDI (Digest) and MALDI (TOF- TOF of MS/MS).
  • Protein separation service (Material production service)
  • Standardized imaging of 2D gel (Material analysis service)
  • Trypsin Digestion (Material processing service)
  • Validation of proteomics data -Validation via RT-PCR, western blot, flow cytometry (FACSCanto II instrument) and immunofluorescence
  • Workshops may be arranged

 

Contact us for services and fees not listed below.

 

Quality Control

Data quality is ensured through the use of appropriate internal and external standards.  The MALDI-TOF mass spectrometers are calibrated with external standards that vary depending on the analyte molecular weight.  This results in the best calibration for the most accurate mass assignments.  Samples that have been trypsin digested are internally recalibrated from observed trypsin autolysis fragments.  The electrospray mass spectrometers are calibrated using polyalanine which allows for even distribution of multiple mass peaks. A variety of search engines, for protein indentification from mass fingerprinting and for intrepretation of MS/MS spectra, are available each of which generates its own statistics.  The choice of database searching software to be applied is optional to investigators and discussion of this matter is encouraged with our technical support team.