LTQ Orbitrap Velos (Thermo Scientific)

 

LTQ Orbitrap Velos is the next generation mass spectrometers, thanks to its significantly increased sensitivity and scan speed.  This is achieved by vacuum interface using a stacked ring radio frequency ionguide with 10-fold higher transfer efficiency in MS/MS mode and 3-5 fold in full scan spectra, by a dual pressure ion trap configuration, and by reduction of overhead times between scans.  The first ion trap efficiently captures and fragments ions at relatively high pressure whereas the second ion trap realizes extremely fast scan speeds at reduced pressure.  Ion injection times for MS/MS are predicted from full scans instead of performing automatic gain control scans.  Together these improvements routinely enable acquisition of up to ten fragmentation spectra per second. Furthermore, an improved higher-energy collisional dissociation cell with increased ion extraction capabilities was implemented.  Higher-collision energy dissociation with high mass accuracy Orbitrap readout is as sensitive as ion trap MS/MS scans in the previous generation of the instrument.  Further, this configuration is supplied with ETD module, which preserves protein labile modification.